EXPERIMENTAL STRATEGY ASSOCIATED WITH SUCROSE HYDROLYSIS OPTIMIZATION

Abstract

Introduction:
Taking advantage of the need to increase hydrolysis efficiency and seeking a sustainable alternative for glucose production, a study was developed focused on optimizing the enzymatic hydrolysis of sucrose by incorporating Komagataella dead cell biomass.
Objective:
To evaluate the influence of operational variables on the yield of enzymatic hydrolysis, using the retained activity in the cellular periplasm.
Materials and Methods:
A Plackett-Bürman factorial design was used to evaluate five variables (temperature, Brix degrees, enzyme concentration, biomass/supernatant ratio, and stirring speed) on the total reducing sugar concentration (g/L) and the percentage of inversion. Quantification was performed using the 3,5-dinitrosalicylic acid (DNS) method. The experiment was conducted at laboratory scale, controlling reaction conditions for subsequent statistical analysis.
Results and Discussion:
Results indicate that Brix degree and the Biomass/Supernatant Ratio are statistically significant (85 %). The model confirmed the positive influence of both variables. The maximum concentration of total reducing sugars (1020 g/L) was achieved with 75 % Brix and a 70/30 mL/g ratio. The necessity of balancing hydrolysis yield with the viability of subsequent operations was discussed.
Conclusions:
The integration of biomass constitutes an effective strategy that enhances catalytic efficient and sustainable alternative to improve glucose production while reducing operating costs. The biomass/supernatant ratio and Brix degrees are the critical operating parameters for process optimization.