LIPASE PRODUCTION IN SOLID FERMENTATION WITH ASPERGILLUSNIGER: PH INFLUENCE

Abstract

Obtaining lipases by solid-state fermentation using Aspergillus niger has been a promising process. However, is necessary to determine the most appropriate pH conditions using a medium based on national origin agroindustrial residues. An experiment in which this factor was studied, as well as fermentation time in five levelswas designed. The response variables to be measured were enzyme activity and productivity. As result, it was achieved that the maximum productivity values were reached at low initial pH and lower fermentation times. The initial pH of 5 had the highest activity and productivity observed. The maximum productivity at pH 5 was 1.4 IU / hr, achieved at 72 hours, with a lipolytic activity of 210.11 IU / gMS.